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Test Code AP Flow Bld Flow Cytometry Blood or CD4:CD8 Ratio (CD4 Count)

Important Note

Cerner Order Code: Pathology Flow Cytometry Request, Blood

Under comment list tests ordered: Flow Cytometry or CD4:CD8 Ratio (CD4 Count)

Note: West Virginia state law requires a patient consent for HIV related testing.

Performing Laboratory

Memorial Hospital Laboratory- Special Hematology   304-388-9511

Test Profile

Report includes patholgoy interpretation for Flow Cytometry or CD3, CD4, CD8 and an interpretation.

Specimen Requirements

Whole Blood-Lavendar Top (EDTA) and Yellow Top (ACD[solution B]) Tube 

5 mL -EDTA and 10 mL -ACD (solution B). Do not transfer blood to other containers.

 

1. Collection date and time are required on request form for processing.

2. A pathology/diagnostic report, name and telephone number of ordering physician, and a brief history are essential to achieve a consultation fully relevant to ordering physician’s needs.

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient
  • Clotted specimens 

Storage and Stability

Room Temperature: 24 hours

Specimen cannot be refrigerated or frozen.

Reference Values

CD3+ 53-93% 359-2,479 cells/mcL
CD3+/CD4+ 24-64% 400-1404 cells/mcL
CD3+/CD8+ 14-38% 199-767 cells/mcL
CD4:CD8 Ratio 1.5-2.5%  

Methodology

Flow Cytometry

Days Test Set Up and Turnaround

Monday through Thursday 8AM-4PM

Friday 8AM-12PM

CPT Coding

88184-First Marker

88185-Each Additional Marker

88187-Pathology Interpretation