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Test Code Amnisure ROM PAMG-1/ Placental a-1 microglobulin (Rupture of Membrane)

Important Note

Contact Women and Children's Laboratory for collection vials

Do not transport samples on ice

Performing Laboratory

Women and Children's Hospital Laboratory 304-388-2385
Greenbrier Valley Laboratory 304-647-6060

Specimen Requirements

Vaginal Swab placed in solvent from the Aminsure Kit

  1. Take the solvent vial by its cap and shake well to make sure all liquid in the vials has dropped on the bottom.  Open the solvent vial and put it in a vertical position. 
  2. To collect a sample from the surface of the vagina use the sterile polyester swab provided.  Remove the sterile swab from its package following instructions on the package.  The polyester tip should not touch anything prior insertion into the vagina.  Hold the swab in the middle of the stick and, while the patient is lying flat on her back, carefully insert the polyester tip of the swab into the vagina until the fingers contact the skin no more than 2-3 inches (5-7 cm) deep. 
  3. Withdraw the swab from the vagina after 1 minute.
  4. Place the polyester tip into the vial and rinse the swab in the solvent by rotating for one minute. 
  5. Remove then dispose of the swab. 

Specimen Rejection

  • Improperly labeled, collected, stored, and transported

Storage and Stability

Room Temperature 30 min
Refrigerate 6 hrs

Reference Values

Negative-No membrane rupture

Methodology

Qualitative Immunochromatographic assay

Days Test Set Up and Turnaround

Monday through Sunday, Continuously

Stat                Expected within 2 hrs       

CPT Coding

84112

Additional Information

When there is a significant presence of blood on the swab, the test can malfunction and is not recommended.  In cases of only trace amounts of blood on the swab, the test still functions properly.
In very rare cases when a sample is taken 12 hours or later after a rupture, a false negative result may occur due to the obstruction of fetus or resealing of the amniotic sac.AmniSure should  not be used earlier than 6 hours after the removal on any disinfectant solution or medicines from the vagina.Results should always be used in conjunction with other clinical information. Women may labor spontaneously despite a negative test result. Placenta previa, and performing digital exams prior to sample collection can lead to inaccurate test results. Failure to detect membrane rupture does not assure the absence of membrane rupture. The performance of the AmniSure test has not been established in the presence of meconium in the amniotic fluid. False negative results and delay in the diagnosis of rupture of membranes can increase the risk of chorioamnionitis, oligohydramnios and {fetal} umbilical cord accident.The performance of AmniSure test has not been established in the presence of the following contaminants: meconium, anti-fungal creams or suppositories, K-Y Jelly, Monistat, Baby Powder (starch and talc), Replens, or Baby Oil. Interrupted leakage with minimal residual fluid can lead to false negative results. Until the diagnosis of membrane rupture is excluded, avoid digital cervical examination to prevent infection and shorten the latency period.