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HelpTest Code B6PRO Vitamin B6 Profile (Pyridoxal 5-Phosphate and Pyridoxic Acid), Plasma
Shipping Instructions
Ship specimen in amber vial to protect from light.
Specimen Required
Patient Preparation:
1. Patient should fast overnight (12-14 hours); infants-should have specimen collected before next feeding. Water can be taken as needed.
2. For 24 hours before specimen collection, patient must not take multivitamins or vitamin supplements.
Supplies: Amber Frosted Tube, 5 mL (T915)
Collection Container/Tube: Green top (sodium or lithium heparin) or plasma gel separator (PST)
Submission Container/Tube: Amber vial
Specimen Volume: 1 mL
Collection Instructions:
1. Centrifuge at 4° C within 2 hours of collection.
2. Aliquot all plasma into amber vial and freeze immediately.
Useful For
Determining vitamin B6 status, including in persons who present with progressive nerve compression disorders, such as carpal tunnel and tarsal tunnel syndromes
Determining the overall success of a vitamin B6 supplementation program
Diagnosis and evaluation of hypophosphatasia
Profile Information
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| PLP | Pyridoxal 5-Phosphate (PLP), P | Yes | Yes |
| B6PA | Pyridoxic Acid (PA), P | No | Yes |
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Vitamin B6 Profile (PLP and PA), PSpecimen Type
Plasma HeparinSpecimen Minimum Volume
0.75 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Heparin | Frozen | 29 days | LIGHT PROTECTED |
Reference Values
PYRIDOXAL 5-PHOSPHATE
5-50 mcg/L
PYRIDOXIC ACID
3-30 mcg/L
Day(s) Performed
Monday through Thursday, Saturday, Sunday
Performing Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
82542
84207
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| B6PRO | Vitamin B6 Profile (PLP and PA), P | 95266-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 61065 | Pyridoxic Acid (PA), P | 1688-1 |
| 4047 | Pyridoxal 5-Phosphate (PLP), P | 30552-4 |