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Test Code C Anaerob Anaerobic Bacteria Culture

Important Note

Includes Gram Stain and Reflex testing ordered as appropiate

Extreme care must be taken to avoid contamination by anaerobic normal flora and overgrowth by coexisting aerobic bacteria.  

Performing Laboratory

Memorial Hospital -Microbiology Laboratory 304-388-9353
Greenbrier Valley Laboratory 304-647-6060

Specimen Requirements

1. Submit specimen in an anaerobic transport vial supplied
2. Label vial with patient’s name (first and last), test requested, specimen source, and date and time of collection.
3. Specimen source is required on request form for processing.

 

Specimen Type Collection Instructions
Any Closed Abcess Aseptically aspirate 0.5 mL of specimen using a needle and syringe
Body Fluid Aseptically collect 0.5 mL of aspirated fluid
Lower Respiratory Tract Secretions Aseptically collect 0.5 mL of lower respiratory secretion by percutaneous transtracheal aspiration
Sinus Tract/Uterine Cavity/Wound (Deep) Aseptically collect 0.5 mL of specimen by syringe aspiraton using a plastic intravenous type of catheter threaded into the infected site
Swab Specimen Aseptically collect specimen from abcessed cavity, and immediately place in a single anaerobic transport vial 
Tissue Aseptically collect tissue by surgical excision
Urine Aseptically collect 0.5 mL of urine by suprapubic needle aspiration of the bladder

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient
  • Any specimen exposed to air for an extended period of time or not in proper transport media or device.
Head and Neck Throat and nasophyngeal swabs, gingival swabs, superficial material collected by swab
Lungs Expectorated sputum, induced sputum, endotracheal aspirates,unprotected bronchoscopic specimens
Central Nervous System CSF fluids, aerobic swab specimens
Urinary Tract Voided or catheterized urine
Genital Tract Vaginal or cerical swabs
Bone, Joint, or Soft Tissue Superficial material collected by swab
Large Bowl Fecal swab or any specimen containing fecal material
Abdomen Aerobic swab specimens

Storage and Stability

Deliver specimen to lab at ambient temperature

Specimen should be examined as soon as possible.  Store at 4o C if delayed.

Methodology

Conventional Culture Technique

Days Test Set Up and Turnaround

Monday through Sunday

CPT Coding

87075-culture, anaerobic

87205-Gram stain