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Test Code DIFF Differential (WBC)

Important Note

Mixed specimen throughly by gentle inversion (8-10 times) immediately after collection

Differential cannot be ordered as a separate test, it is included in a CBC.

Performing Laboratory

Memorial Hospital Laboratory- Automated Procedures  304-388-5953       
General Hospital Laboratory 304-388-6244
Women and Children's Hospital Laboratory 304-388-2385
Teays Valley Hospital Laboratory 304-757-1770
Plateau Medical Center Laboratory 304-469-8621
Greenbrier Valley Laboratory 304-647-6060

Specimen Requirements

Whole Blood-Lavender-top (EDTA) tube or microtainer

3 mL of  whole blood (minimum 1 full microtainer)

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient
  • Clotted specimen
  • Grossly hemolyzed
  • Contaminated

Storage and Stability

Blood smears should be made on specimens less than 4 hours old.  The longer the blood is stored, the more distortion of cells will occur.

Reference Values

    Male Female Male Female M/F M/F M/F
    >18yo >18yo 10-18yo 10-18yo 2-10yo 1mon-2yr 0-1 month
Segs % 50-78 50-78 33-97 38-92 32-91 19-87 24-82
Lymph % 20-35 20-35 4-51 8-49 7-55 10-67 12-62
Monos % 0-8 0-8 3-15 3-13 3-15 5-16 2-16
Eos % 0-4 0-4 0-6 0-6 0-7 0-5 0-8
Baso % 0-2 0-2 0-2 0-2 0-4 0-3 0-3

Methodology

Light microscope

Days Test Set Up and Turnaround

Monday through Sunday, Continuously

AM Collection Expected by 9AM same day
Routine Expected within 24 hrs
Stat Expected within 1 hr
Timed Expected results are test dependant

CPT Coding

85007