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Test Code EPU Electrophoresis, Protein, 24 Hour, Urine


Shipping Instructions


Refrigerate specimen during collection and send refrigerated.



Necessary Information


24-Hour volume is required.



Specimen Required


Supplies: Aliquot Tube, 5 mL (T465)

Submission Container/Tube: Plastic, 60-mL urine bottle and plastic, 5-mL tube

Specimen Volume: 50 mL

Collection Instructions:

1. Collect urine for 24 hours.

2. Aliquot specimen between containers.

3. Label specimens appropriately (60-mL bottle for protein electrophoresis and 5-mL tube for protein, total).

Additional Information: See Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens in Special Instructions for multiple collections.


Useful For

Monitoring patients with monoclonal gammopathies

Profile Information

Test ID Reporting Name Available Separately Always Performed
PTU Protein, Total, U Yes Yes
PEU Protein Electrophoresis, U No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IFXU Immunofixation, U No No

Testing Algorithm

Urine protein electrophoresis alone is not considered an adequate screening for monoclonal gammopathies.

 

If a discrete electrophoresis band is identified, the laboratory will evaluate the urine protein electrophoresis and, if necessary, perform immunofixation at an additional charge.

 

The following algorithms are available in Special Instructions:

-Laboratory Approach to the Diagnosis of Amyloidosis

-Laboratory Screening Tests for Suspected Multiple Myeloma

Method Name

PTU: Turbidimetry

PEU: Agarose Gel Electrophoresis

IFXU: Immunofixation

Reporting Name

Electrophoresis, Protein, U

Specimen Type

Urine

Specimen Minimum Volume

25 mL

Specimen Stability Information

Specimen Type Temperature Time
Urine Refrigerated (preferred) 14 days
  Frozen  5 days
  Ambient  72 hours

Reference Values

PROTEIN, TOTAL

<229 mg/24 hours

 

Reference values have not been established for patients <18 years of age.

Reference value applies to 24-hour collection. 

 

ELECTROPHORESIS, PROTEIN

The following fractions, if present, will be reported as a percent of the protein, total:

Albumin

Alpha-1-globulin

Alpha-2-globulin

Beta-globulin

Gamma-globulin

Day(s) and Time(s) Performed

Protein, total: Monday through Sunday; Continuously

Electrophoresis, protein: Monday through Saturday; 12 p.m.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

84156

84166

86335-Immunofixation (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EPU Electrophoresis, Protein, U In Process

 

Result ID Test Result Name Result LOINC Value
2776 Albumin 13986-5
TP2 Total Protein 2889-4
TM23 Collection Duration 13362-9
2777 Alpha 1-Globulin 13984-0
2779 Alpha 2-Globulin 13987-3
VL21 Urine Volume 19153-6
CONC1 Concentration 35663-4
2780 Beta-Globulin 13988-1
2781 Gamma-Globulin 13989-9
2833 A/G Ratio 44294-7
21446 M spike 42482-0
22307 M spike 42482-0
21447 Impression 32210-7

Urine Preservative Collection Options

Note: The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.

Ambient

Yes

Refrigerated

Preferred

Frozen

Yes

6N HCl

No

50% Acetic Acid

No

Na2CO3

No

Toluene

Yes

6N HNO3

No

Boric Acid

No

Thymol

Yes