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Lactic Acid, Plasma

Performing Laboratory

Charleston Area Medical Center, Inc.

Methodology

                                          LDH

L-lactate + NAD+ ---------------------->  Pyruvate + NADH + H+

 

The absorbance due to NADH is directly proportional to the lactate concentration and is measured using a two-filter (340 - 383 nm) end point technique.

Specimen Requirements

Container - Na-fluoride/K oxalate (Gray top tube)

Plasma  2 ml.   Collection of a satisfactory specimen for lactate analysis requires special procedures to prevent changes in lactate while and after the specimen is drawn.  The patient should be fasting and at complete rest.    Blood is best collected without stasis in a container of sodium fluoride/potassium oxalate, followed by immediate chilling of the specimen and separation of the cells within 15 minutes.     Analyze specimen as soon as possible.

Reference Values

0.4 - 2.0 mmol/L  (3.6 - 18.00 mg/dL)

Test Classification and CPT Coding

83605

Day(s) Test Set Up

Monday - Sunday