Sign in →

Test Code MTX Methotrexate

Important Note

Collect on ice and protect from light

Performing Laboratory

Women and Children's Hospital Laboratory              304-388-2385           

Specimen Requirements

Dark Green Lithium Heparin

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient

Storage and Stability

Refrigerated 2-8oC 2 weeks
Frozen <-20oC Undetermined

Reference Values

Methotrexate levels in excess of 50 mcmol/L at 24 hours, 10 mcmol/L at 48 hours, and 0.5 mcmol/L at 72 hours potential toxicity.  Methotrexate values should be used in conjunction with information available from clinical evalution and other diagnostic procedures.  NOTE:  This assay should not be used if patient is receiving possible compassionate therapy with glucarpidase.

Methodology

Homogeneous competitive enzyme immunoassay

Days Test Set Up and Turnaround

Monday through Sunday, Continuously 

AM Collection Expected by 9AM same day
Routine Expected within 24 hrs
Stat Expected within 1 hr
Timed Expected results are test dependant

CPT Coding

80299