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Test Code STL Enteric Pathogens Culture, Feces


Advisory Information


1. If Vibrio is suspected, order VIBC / Vibrio Culture, Stool.

2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC. Pathogens for which susceptibility testing would be pursued are: Shigella species, Yersinia species, Campylobacter species, Arcobacter species, and Vibrio species. If susceptibilities are not appropriate they will not be performed and ZMMLS will be canceled at report time.

3. Clostridioides (Clostridium) difficile, a major cause of nosocomial diarrhea, is not detected by this test (order CDFRP / Clostridioides (Clostridium) difficile Toxin, Molecular Detection, PCR, Feces).

4. Escherichia coli O157:H7 is not detected by this test (Order STFRP / Shiga Toxin, Molecular Detection, PCR, Feces).



Additional Testing Requirements


In some cases, local public health requirements may impact Mayo Clinic Laboratories (MCL) clients and require submission of isolates to a public health laboratory. Clients should familiarize themselves with local requirements and will be responsible for submitting isolates to the appropriate public health laboratory. Clients can obtain isolates of Vibrio species by calling 800-533-1710 as soon as possible to ensure viability of the organism.



Shipping Instructions


Specimen must arrive within 96 hours of collection.



Necessary Information


Specimen source is required.



Specimen Required


Patient Preparation: Medications: Do not use antacids, barium, bismuth, antidiarrheal medication, or oily laxatives before collection of specimen.

Supplies: C and S vial (T058)

Specimen Type: Preserved Feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S [T058])

Specimen Volume: Representative portion of fecal specimen

Collection Instructions:

1. Collect fresh feces and submit 1 gram or 5 mL in container with transport medium.

2. Place feces in preservative within 2 hours of collection.

3. Place vial in a sealed plastic bag.


Forms

If not ordering electronically, complete, print, and send a Gastroenterology and Hepatology Client Test Request (T728) with the specimen.

Useful For

Determining whether a bacterial enteric pathogen is the cause of diarrhea

 

May be helpful in identifying the source of the infectious agent (eg, dairy products, poultry, water, or meat)

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
GID Bacteria Identification No, (Bill Only) No
ISAE Aerobe Ident by Sequencing No, (Bill Only) No
REFID Additional Identification Procedure No, (Bill Only) No
SALS Serologic Agglut Method 1 Ident No, (Bill Only) No
SHIG Serologic Agglut Method 3 Ident No, (Bill Only) No
RMALD Ident by MALDI-TOF mass spec No, (Bill Only) No

Testing Algorithm

When this test is ordered, the reflex tests may be performed and charged separately. Reportable pathogens include: Aeromonas, Campylobacter, Salmonella, Shigella, and Yersinia.

Note: Does not include culture for Escherichia coli O157:H7. All bacterial isolates worked up, to rule in or out as a pathogen, will be billed for work performed and resources consumed.

 

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Method Name

Conventional Culture Technique

Reporting Name

Enteric Pathogens Culture, Stool

Specimen Type

Fecal

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Fecal Ambient (preferred) 4 days
  Refrigerated  4 days

Reference Values

No growth of pathogens

Day(s) and Time(s) Performed

Monday through Sunday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87045-Enteric Pathogens Culture, Stool-with isolation and preliminary examination

87046 x 3-Stool Culture Aerobic Bacteria, each

87077-Ident by MALDI-TOF mass spec (if appropriate)

87077-Bacteria Identification (if appropriate)

87153-Aerobe Ident by Sequencing (if appropriate)

87077-Additional Identification Procedure (if appropriate)

87147 x 3-Serologic Agglut Method 1 Ident (if appropriate)

87147 x 4-Serologic Agglut Method 3 Ident (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
STL Enteric Pathogens Culture, Stool 625-4

 

Result ID Test Result Name Result LOINC Value
STL Enteric Pathogens Culture, Stool 625-4