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Test Code TOBRA Peak Tobramycin, Peak

Important Note

Samples containing amikacin or kanamycin cannot be reliably quantitated by this assay.

Beta-lactam antibiotics may inactivate tobramycin.

Patient's taking gentamicin may cross react with the tobramycin assay.

Standard dose of tobramycin does not always yield a predictable serum/plasma level because the drug's concentration also depends on the patient's volume of distribution, drug elimination, mode of administration, volume of extracellular fluid, renal function and physiological changes during therapy

Performing Laboratory

Memorial Hospital Laboratory- Automated Procedures  304-388-5953       
Greenbrier Valley Laboratory 304-647-6060

Collection and Processing

Peak samples should be drawn 30-60 minutes after intravenous infusion or 60-90 minutes after intramuscular injection.

Specimen Requirements

Preferred: Plasma-Dark Green Lithium Heparin or Serum- red top

Acceptable: Plasma-Mint Green or Serum-SST gold top

Allow SST or red top to completely clot      

Spin and physically separate plasma/serum from contact with cells as soon as possible

4 mL of plasma/serum (minimum 2 mL)

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient

Storage and Stability

Specimens should be separated and tested immediately after collection or specimen should be separated and frozen.

Reference Values

Therapeutic Range

Peak 5.0-10.0 mcg/mL

Critical Values

Peak > 12.0 mcg/mL

Methodology

Homogeneous competitive enzyme immunoassay Memorial Automated Procedure Lab
Two-Point Rate Greenbrier 

Days Test Set Up and Turnaround

Monday through Sunday, Continuously

AM Collection Expected by 9AM same day
Routine Expected within 24 hrs
Stat Expected within 1 hr
Timed Expected results are test dependant

CPT Coding

80200