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Test Code VIT B12 Vitamin B12 Assay

Performing Laboratory

Beckley Cancer Center Laboratory 304-461-2222
Greenbrier Valley Laboratory 304-647-6060
Memorial Hospital Laboratory- Automated Procedures  304-388-5953       
Parkersburg Laboratory 304-865-3679
Teays Valley Hospital Laboratory 304-757-1770

Specimen Requirements

Plasma- Mint Green, Dark Green Lithium Heparin or Serum- SST gold top, red top

Allow SST or red top to completely clot

Spin and physically separate plasma/serum from contact with cells as soon as possible

4 mL of plasma/serum (minimum 2 mL)

 

Additional sample type that can be used ONLY at Beckley, Greenbrier, and Parkersburg

EDTA plasma

Specimen Rejection

  • Improperly labeled, collected, stored, and transported
  • Quantity not sufficient
  • Hemolyzed 

Additional rejections ONLY at Beckley, Greenbrier, and Parkersburg 

  • Turbid
  • Lipemic

Storage and Stability

Memorial and Teays Only 

Room temperature 15-30oC 8 hrs
Refrigerated 2-8oC 24 hrs
Frozen <-20oC Not specified

Additional storage and stability ONLY at Beckley, Greenbrier, and Parkersburg

Refrigerated 2-8oC 7 days
Frozen <-20oC 4 weeks

Avoid repeated freeze-thaw cycles.

Reference Values

180.0-914.0 pg/mL

Methodology

Memorial and Teays Chemiluminescent  immunoenzymatic competitive assay
Beckley, Greenbrier, Parkersburg Competitive binding immunoassay

Days Test Set Up and Turnaround

Greenbrier, Memorial, Teays-Monday through Sunday, Continuously

Beckley, Parkersburg-Monday through Friday

AM Collection Expected by 9AM same day
Routine Expected within 24 hrs
Stat Expected within 1 hr
Timed Expected results are test dependant

CPT Coding

82607