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Test Code VLTB Volatile Screen, Blood


Ordering Guidance


This test is not performed using chain of custody. For chain of custody testing, order VLTBX / Volatile Screen, Chain of Custody, Blood.



Additional Testing Requirements


If measurement of ethylene glycol is also needed, order ETGL / Ethylene Glycol, Serum.



Specimen Required


Container/Tube:

Preferred: Gray top (potassium oxalate/sodium fluoride)

Acceptable: Lavendar top (EDTA) or green top (sodium heparin)

Specimen Volume: 2 mL

Collection Instructions: Do not use alcohol to clean arm. Use alternatives such as Betadine to cleanse arm before collecting any specimen for volatile testing.


Useful For

Detection and quantitation of acetone, methanol, isopropanol, and ethanol in whole blood

 

Quantification of the concentration of ethanol in blood that correlates with the degree of intoxication

 

Evaluation of toxicity to the measured volatile substances

 

This test is not intended for use in employment-related testing.

Testing Algorithm

This test includes analysis of methanol, ethanol, isopropanol, and acetone.

Method Name

Headspace Gas Chromatography Flame Ionization Detector (HSGC-FID)

Reporting Name

Volatile Scrn, B

Specimen Type

Whole blood

Specimen Minimum Volume

0.5 mL or amount to fill 1 tube

Specimen Stability Information

Specimen Type Temperature Time Special Container
Whole blood Refrigerated (preferred) 14 days
  Frozen  28 days
  Ambient  24 hours

Reference Values

Methanol:

Not detected (Positive results are quantitated.)

Toxic concentration: ≥10 mg/dL

 

Ethanol:

Not detected (Positive results are quantitated.)

Toxic concentration: ≥400 mg/dL

 

Isopropanol:

Not detected (Positive results are quantitated.)

Toxic concentration: ≥10 mg/dL

 

Acetone:

Not detected (Positive results are quantitated.)

Toxic concentration: ≥10 mg/dL

Day(s) Performed

Monday through Saturday

Report Available

1 to 2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

80320

G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VLTB Volatile Scrn, B 41266-8

 

Result ID Test Result Name Result LOINC Value
89190 Volatile Scrn, B 41266-8
30921 Methanol, B 9334-4
30922 Ethanol, B 5640-8
30923 Acetone, B 9425-0
30924 Isopropanol, B 5667-1
34377 Chain of Custody 77202-0

Forms

If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.